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cyflow space flow cytometer  (Partec)

 
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    Partec cyflow space flow cytometer
    Cyflow Space Flow Cytometer, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyflow space flow cytometer/product/Partec
    Average 90 stars, based on 1 article reviews
    cyflow space flow cytometer - by Bioz Stars, 2026-03
    90/100 stars

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    FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow <t>cytometry</t> (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
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    cyflow® space cytometer  (Partec)
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    FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

    Journal: ACS Catalysis

    Article Title: Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay

    doi: 10.1021/acscatal.5c01903

    Figure Lengend Snippet: FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

    Article Snippet: Flow cytometry analysis was conducted with a Partec CyFlow space device (Sysmex Partec GmbH, Görlitz, Germany).

    Techniques: Activity Assay, Fluorescence, Expressing, Negative Control, Flow Cytometry